Desulfovibrio desulfuricans ist ein sulfatreduzierendes bakterium. Proton translocation by washed cells of the sulfatereducing bacterium desulfovibrio desulfuricans strain essex 6 was studied by means of ph and sulfide electrodes. An examination of conditions for the growth of desulfovibrio desulfuricans, with the aim of optimizing hydrogenase production, is reported. In this study, we conducted experiments with the hgmethylating bacterium desulfovibrio desulfuricans nd2 to elucidate the role of cellular thiols in anaerobic hg0 oxidation. The reported case of a desulfovibrio desulfuricans bacteremia occurring in an immunocompromised host with ulcerative colitis confirms that this organism may be a possible. Desulfovibrio sulfodismutans, and desulfovibrio desulfuricans kramer and cypionka, 1989. Canadian journal of microbiology canadian science publishing. A multiplex pcr was devised for the identification of desulfovibrio piger formerly desulfomonas pigra, desulfovibrio fairfieldensis and desulfovibrio desulfuricans, and applied to the above isolates. An ammonium sulfate lactate yeast extract medium gave 5 to 10 times as much hydrogenase activity as a peptone yeast extract medium. The toxic effects of uvi were studied using desulfovibrio desulfuricans g20 in a medium containing bicarbonate or 1,4.
Pdf sulfatereducing bacterium desulfovibrio desulfuricans. Sulfide product inhibition of desulfovibrio desulfuricans in batch and. Uvi was reduced to uiv when washed cells of sulfategrown desulfovibrio desulfuricans were suspended in a bicarbonate buffer with lactate or h2 as the electron donor. Pdf this article aims to study hydrogen productionconsumption in desulfovibrio d. Desulfovibrio desulfuricans and correlate it with the composition of extracellular polymeric substances eps of the biofilm formed by these bacteria. Formate dehydrogenase from desulfovibrio desulfuricans atcc.
Desulfovibrio article about desulfovibrio by the free. Desulfovibrio strains have been found in a variety of habitats, including soil, the intestines and feces of animals, and both salinic and fresh water. Characterization of palladium nanoparticles produced by healthy and microwaveinjured cells of desulfovibrio desulfuricans and escherichia coli by jaime gomezbolivar 1, iryna p. Desulfovibrio desulfuricans beijerinck, 1895 kluyver and van niel, 1936. R smithbergeys manual of determinative bacteriology. The rcsb pdb also provides a variety of tools and resources. Growth of desulfovibrio desulfuricans was investigated under heterotrophic and anaerobic conditions. Uraniumvi toxicity was dependent on the medium buffer and was observed in terms of longer lag times and, in some cases, no.
The objective of this study was to demonstrate the ability of the sulfatereducing bacterium, desulfovibrio desulfuricans, to reduce movi to moiv in anaerobic environments. At ph 5, cell population densities decreased signi. Patel for help with elec tron microscopy, gas chromatography and dna. Corrosion of iron and formation of iron phosphide by. Desulfovibrio is a genus of gramnegative sulfatereducing bacteria. Abdollahi and wimpenny stated that some strains of d. Several studies have shown that dom can enhance hg methylation, especially under sulfidic conditions, whereas others show that dom inhibits hg methylation due to strong hgdom complexation. Reversible extrusion of protons could be induced either by addition of electron acceptors to cells incubated under hydrogen, or by addition of hydrogen to cells incubated in the. Phylogenetic studies indicated that strain dsm 1925 t was distantly related to the type strain of desulfovibrio vulgaris 95. Several groups of researchers have reported desulfovibrio like organisms, based on 16s rdna sequence similarity, associated with proliferative bowel diseases from ferrets.
In this study, we investigated and compared the effects of dom on hg. Desulfovibrio desulfuricans aestuarii postgate and campbell, 1966 subspecies. Desulfovibrio and desulfuromonas reduce sulfate and elemental sulfur s, respectively, yielding sulfide s2. Sulfatereducing bacteria srb have been implicated in the etiopathogenesis of ulcerative colitis because of their ability to produce hydrogen sulfide, which might lead to. The pd nanoparticles were small and largely monodispersed, between 0. These organisms infrequently cause human infections, and the clinical characteristics of infection with desulfovibrio spp.
Pdf hydrogen metabolism in desulfovibrio desulfuricans strain. Desulfovibrio desulfuricans an overview sciencedirect topics. The strains of sulfatereducing bacteria consisted of d. Sequencing of sulfonatemetabolizing anaerobes is key for understanding sulfonate degradation and its role in the sulfur cycle. Bacteria of desulfovibrio desulfuricans species are sulphatereducing gramnegative rods settling ecosystems devoid of oxygen. When the corresponding gene in desulfovibrio desulfuricans g20 was knocked out. A note on the production of radioactive sulphide by. Its cells are gramnegative, slightly curved rods gilmour et al. A study on electron transportdriven proton translocation in. Pdf we propose the use of desulfovibrio desulfuricans nd2 as a model species for understanding the mechanism of microbial hg.
Desulfovibrio cells have mesophillic growth patterns with an ideal growth rate between the. During the last 15 years, however, it has turned out that they have manifold properties that enable them to cope with oxygen. Desulfovibrio species are commonly found in aquatic environments with high levels of organic material, as well as in waterlogged soils, and form major community members of extreme oligotrophic habitats such as deep granitic fractured rock aquifers. There was no uvi reduction in the absence of an electron donor or when the cells were killed. Desulfovibrio longus desulfovibrio piger desulfovibrio longreachensis desulfovibrio oxamicus desulfovibrio multispirans. Sulfatereducing bacterium desulfovibrio desulfuricans. Throughout the first 90 years after their discovery, sulfatereducing bacteria were thought to be strict anaerobes. Here, we describe a case of desulfovibrio desulfuricans bacteremia in an 88yearold japanese man with a past medical history of thoracic. Identification of these species with conventional methods is difficult.
The strains benghazi and walvis bay can be distinguished from 40 strains of desulfovibrio and from d. Desulfovibrio desulfuricans an overview sciencedirect. However, desulfovibrio species are capable of growing in the presence of oxygen. Desulfovibrio desulfuricans has been reported to be the cause of a case of gastrointestinal disorder and bacteremia with episodes of fever, chills, and profuse perspiration. Desulfovibrio desulfuricans strain atcc 27774 dsm 6949 is a sulfatereducing bacterium isolated from the rumen of a sheep. It is generally found in soils, waters, and the stools of animals, although in rare cases it has been found to cause infection in humans. Intracellular hg0 oxidation in desulfovibrio desulfuricans. For initial growth to occur, it was found that the eh or redox potential must. Characterization of palladium nanoparticles produced by.
The toxicity of al to desulfovibrio desulfuricans g20 was assessed over a period of 8 weeks in a modi. The possibility that sulfatereducing microorganisms contribute to uvi reduction in sedimentary environments was investigated. Citing literature bergeys manual of systematics of archaea and bacteria. Under anoxic conditions, certain anaerobic bacteria can oxidize dissolved elemental mercury and convert the oxidized hg to neurotoxic methylmercury. While most isolates have not been identified to the species level, desulfovibrio fairfieldensis and d. Extracellular ironsulfur precipitates from growth of. Many sulphate reducing bacteria can also reduce nitrite, but relatively few isolates are known to reduce nitrate. Under these conditions the growth of desulfovibrio desulfuricans was characterized by a maximum growth rate of 25 10. Journal of environmental quality abstract reduction and. For growth on a 2% yeast extractagar surface in the absence of hydrogen nitrogen atmosphere, sodium lactate was required in the medium. Growth of desulfovibrio desulfuricans under heterotrophic and. Toxic effects of uranium on desulfovibrio desulfuricans g20. Reclassification of the sulfate and nitratereducing. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Molybdenumvi was reduced to moiv by washed cells of d. Sulfatereducing bacterium desulfovibrio desulfuricans nd2 as a model for understanding bacterial mercury methylation. Sulfatereducing bacteria not only survive oxygen exposure for at least days, but many of them even reduce oxygen to water. For growth on a 2% yeast extractagar surface in the absence of hydrogen nitrogen atmosphere, sodium lactate was required in the. Growth of desulfovibrio on the surface of agar media. A case of liver abscess with desulfovibrio desulfuricans. The genome sequence of the anaerobic, sulfatereducing. Johns hospital health center, santa monica, california 90404 3. Desulfovibrio desulfuricans bacteremia in a dog journal of. The authors have examined extracellular ironbearing precipitates resulting from the growth of desulfovibrio desulfuricans in a basal medium with lactate as the carbon source and ferrous sulfate. Desulfovibrio desulfuricans beijerinck 1895 kluyver and van niel 1936, 397 spirillum desulfuricans beijerinck 1895, 1.
Natural dissolved organic matter dom affects mercury hg redox reactions and anaerobic microbial methylation in the environment. Desulfovibrio desulfuricans also known as strain nd 2 description and significance. Microbial influenced corrosion due to desulfovibrio desulfuricans. Cytochrome c3 from desulfovibrio desulfuricans atcc 27774 appears to be capable of receiving two protons and two electrons from hydrogenase for transport to the membrane, and converting electronic energy into proton motive force. We propose the use of desulfovibrio desulfuricans nd2 as a model species for understanding the mechanism of microbial hg methylation. Desulfovibrio desulfuricans lipopolysaccharides induce endothelial cell il6 and il8 secretion and eselectin and vcam1 expression. Desulfovibrio africanus desulfovibrio desulfuricans desulfovibrio fructosovorans desulfovibrio gigas desulfovibrio salexigens desulfovibrio vulgaris desulfovibrio sp. There was no uvi reduction in the absence of an electron donor or when the cells.
Jan 15, 2020 desulfovibrio desulfuricans strain atcc 27774 dsm 6949 is a sulfatereducing bacterium isolated from the rumen of a sheep. Desulfovibrio desulfuricans strain nd 2 was isolated from chesapeake bay sediments in may 1985 gilmour et al. Desulfovibrio desulfuricans is a gramnegative sulfatereducing bacteria. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. Effect of a rosmarinic acid supplemented hemodialysis fluid on inflammation of human vascular endothelial cells. The purpose of this paper is to investigate microbially induced corrosion on stainless steels due to sulfate reducing bacteria sp. Alden research laboratory, 1 infectious diseases division, 2 microbiology laboratory, st. Desulfovibrio desulfuricans strain g20 is a spontaneously nalidixic acid resistant derivative of the wildtype strain g100a that was isolated from an oil well corrosion site weimer et al. Desulfovibrio desulfuricans growth kinetics herrera 1991. Growth of desulfovibrio desulfuricans api strain was found to take place in an atmosphere of hydrogen on the agar surface of complex media, including yeast extract difco, and trypticase soy agar bbl without any added reducing agents.
As a member of the wwpdb, the rcsb pdb curates and annotates pdb data according to agreed upon standards. The reported case of a desulfovibrio desulfuricans bacteremia occurring in an immunocompromised host with ulcerative colitis confirms that this organism may be a possible opportunistic human. Desulfovibrio species are commonly found in aquatic environments with high levels of organic material, as well as in waterlogged soils, and form major community members of extreme oligotrophic habitats. Anaerobic oxidation of hydrocarbons by desulfovibrio desulfuricans. Contrasting effects of dissolved organic matter on mercury. The desulfovibrio desulfuricans formate dehydrogenase has been clearly demonstrated to catalyze co 2 reduction using a viologen as reductant, and the reaction has recently been comprehensively examined, with k cat in the reverse direction being determined to be 47 s. Desulfovibrio desulfuricans strain atcc 27774 dsm 6949. Growth of desulfovibrio desulfuricans, under heterotrophic and anaerobic conditions. Although nitrate reductase genes are absent from desulfovibrio vulgaris strain hildenborough, for which the complete genome sequence has been reported, a single subunit periplasmic nitrate reductase, napa, was purified from desulfovibrio desulfuricans strain. It is particularly noted for its ability to produce methyl mercury. Although nitrate reductase genes are absent from desulfovibrio vulgaris strain hildenborough, for which the complete genome sequence has been reported, a single subunit periplasmic nitrate reductase, napa, was purified from desulfovibrio desulfuricans strain 27774, and. Desulfovibrio desulfuricans desulfuricans beijerinck, 1895 kluyver and van niel, 1936. Sulfide product inhibition kinetics for growth and activity of desulfovibrio desulfuricans was investigated in batch and continuous cultures at ph 7. Formate dehydrogenase from desulfovibrio desulfuricans atcc 27774.
1145 1496 515 699 357 194 478 494 162 148 929 335 1212 893 180 596 650 1376 267 1356 1287 1329 320 1097 577 51 1328 5 299 644 986 255 1003 546 1321 1201 733 251 116 4 854 160 964 753 233 1199 669